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SBT Instruments

SBT Instruments builds BactoBox®, a benchtop impedance flow cytometer that measures two things directly from one reading in about two minutes: how many bacterial cells are in the sample, in cells/mL, and how big those cells are. It is used in two applications: microbial process development, batch and fed-batch, at development and pilot scale, where optical density cannot separate more cells from bigger cells and therefore cannot be used to compare cultivations against each other; and inoculum st

For agents: Maker of BactoBox, a benchtop impedance flow cytometer that counts bacterial cells and sizes them in about two minutes for process development and inoculum stan

bacteria cell countingimpedance flow cytometrybactoboxfermentation process monitoringinoculum standardization

Their llms.txt

The beginning of the file, from our validated copy saved 2026-10-23T11:09:12.771Z. Cached copy · Source on sbtinstruments.com

# SBT Instruments

> SBT Instruments builds BactoBox®, a benchtop impedance flow cytometer that measures two things directly from one reading in about two minutes: how many bacterial cells are in the sample, in cells/mL, and how big those cells are. It is used in two applications: microbial process development, batch and fed-batch, at development and pilot scale, where optical density cannot separate more cells from bigger cells and therefore cannot be used to compare cultivations against each other; and inoculum standardization, where the cells are counted before dosing instead of waiting days for a plate count.

SBT Instruments is a Danish company (Herlev, DK) developing instruments for direct measurement of bacterial growth. The flagship product, BactoBox®, uses impedance flow cytometry to count individual bacterial cells in cells/mL and to measure their size, so cell number and cell size can be told apart. Optical density cannot separate the two. The count is a count of whole bacterial cells. It is not a live count, and it does not replace CFU, which measures culturability.

The site is structured around the product, its two applications, in-depth knowledge articles on measuring bacterial growth, and customer stories from fermentation and bioprocess development teams using BactoBox®.

## Product

- [BactoBox® — direct bacterial cell count and cell size](/product): How it works, what the data looks like, and full specifications.
- [Request a demo](/request-demo): Book a call to see BactoBox® on your samples.

## Applications

- [Microbial process development](/applications/microbial-process-development): BactoBox counts cells/mL directly, so process decisions do not rest on assumptions about how OD tracks cells when conditions change.
- [Inoculum standardization](/applications/inoculum-standardization): Dose a known number of viable bacteria. Count your inoculum in about two minutes, instead of waiting one to seven days for a plate count.

## Knowledge

- [Understanding OD600](/knowledge/understanding-od600): What spectrophotometric optical density actually reports, where it holds up, where it misleads, and why it cannot be used to compare one cultivation with another.
- [Understanding BactoBox® cell counts](/knowledge/understanding-bactobox-cell-counts): What a direct count of whole bacterial cells in cells/mL is, how the measurement works, and how it compares with OD600, CFU, microscopy and fluorescent flow cytometry.
- [Nobody measures growth accurately today](/knowledge/nobody-measures-growth-accurately): Real cultivation data where OD600 misleads four routine decisions: the induction point, the mid-run comparison, the media ranking, and when to conclude the process.
- [Are live-dead measurements needed in upstream microbial process development?](/knowledge/are-live-dead-measurements-needed): The rule from the published evidence — if the cell count is rising, any cell death is negligible — why growth cessation precedes cell death, and why
…

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